Thymic and Peripheral Aspects of T cell Aging and Rejuvenation: Human target verification and thymic function analysis
Duke PI: G. Sempowski (NIH/NIA P01AG052359)
Using innovative forward-thinking mouse models and approaches, the Projects in this comprehensive discovery-based Thymus Rejuvenation PPG seek to (i) identify mechanisms behind reduced thymic production and impaired peripheral maintenance of T cells with aging and stress, and (ii) develop combined strategies to ameliorate these defects and improve immune defense against infection and cancer in the elderly. Critical to the translation potential of project-identified mechanisms, pathways and therapeutic targets in future programs, is proof-of-concept verification of applicability of mouse targets/pathways to human thymus aging, and centralized assessment of thymic function performed at Duke in the Duke RBL Immunology Unit.
DOD Center for Long Term Follow-up of the Late Effects of Acute Radiation Exposure in Primates (Project 3: Immune Recovery)
Duke PIs: B, Chen, G. Sempowski (DoD W81XWH-15-1-0574)
This multi-investigator center based at Wake Forest Primate Center investigates the late effects of acute radiation exposure in mice and primates. In Project 3 we hypothesize that both thymopoiesis and peripheral expansion contribute to T cell reconstitution after radiation injury and the relative contribution of these two pathways depends on the dose of radiation. In this project we will: define the roles of thymopoiesis and peripheral expansion in overall T cell reconstitution after radiation-induced injury based on radiation dose; and determine if therapeutic agents identified in our previous studies are able to promote or accelerate overall T cell immunity after radiation injury.
AC STI CRC – Atlantic Coast Sexually Transmitted Infections Cooperative Research Center (Host Response Monitoring Core)
Duke PIs: G. Sempowski, H. Staats (NIH U19-AI113170)
The goals of the Uniformed Services University of the Health Sciences-based AC STI CRC (A. Jerse) are to further the understanding of the immunobiology of gonorrhea, chlamydial infection, and gonorrhea/chlamydial coinfections with the aim of developing novel immunotherapies and a gonorrhea vaccine. The Duke RBL Immunology Unit was awarded the Host Response Monitoring Core to provide centralized, comprehensive and consistent immune monitoring (humoral, cellular and multiplex biomarker assays; Human and Mouse studies)
RadCCORE – Radiation Countermeasures Center of Research Excellence (Immune Monitoring Core)
Duke PIs: N. Chao, D. Kirsch, B, Sullenger, B. Chen, G. Sempowski (NIH U19-AI067798)
The RadCCORE based at Duke supports an Immune Monitoring Core comprised of technologies and services offered by two service laboratories within the Duke Human Vaccine Institute (DHVI): the DHVI Flow Cytometry Facility and RBL Immunology Unit. The Immune Monitoring Core centrally provides RadCCORE investigators with high quality, state-of-the-art cell sorting, multiplex cytokine/chemokine assays, and T cell receptor gene expression analysis for their basic and applied research efforts.