DHVI Flow Cytometry

The DHVI Research Flow Cytometry Facility serves the analytical and cell sorting needs of the Duke Human Vaccine Institute and researchers throughout the Duke Community. DHVI Flow offers state-of-the-art cytometric support to investigators in basic, developmental, and clinical research.

The Flow Cytometry Facility instruments are capable of performing BSL-1 through BSL-3 live cell sorting, phenotypic acquisition, DNA cell cycle analysis, and intracellular marker analysis. Analytical and sorting capabilities up to 18 simultaneous parameters enable researchers to define subpopulations based on cell surface morphology as well as size and complexity. We have two cell sorters (BD FACS Arias) that are operated by facility staff.  We have four cell analyzers (1 BD LSRII, 1 BD Fortessa,1 Luminex Muse and 1 iQue Screener PLUS) available 24/7 for trained users. Instruments are located in the RP105, MSRBII, and GHRB (RBL at Duke).

Download a poster here

Announcements 

Upcoming and Recorded Webinars

 

Recorded Webinars: 

Open Flow: 

Intro to Cell Sorting Part I: https://www.youtube.com/watch?v=P6e9EbNdS64

Intro to Cell Sorting Part II: https://youtu.be/Y39MXA0Bmz4

Intro to Cell Sorting Part III: https://youtu.be/_Iy27WXf5Zk

Open Flow: Flourescence Compensation in Diva, reviews the principles behind compensation and how it is carried out on a BD Fortessa analyzer, using a live-run with a four color experiment.  A recording of this session can be found on their YouTube.) 

Open Flow: Multicolor Troubleshooting in Diva, focuses on rules of compensation and how our data can suffer when we do not follow them. A recording of this fourth session can be found on their YouTube

***DHVI Flow Cytometry Shared Resource 2020 FlowJo Training Workshop, Day 1, Basic: Recording posted here*** (Passcode is g7=yETgZ).  Topics covered will include loading data, accessing metadata, cleaning data through exclusion gates, compensation, keywords, creating groups, creating gates, applying statistics, working the layout and table editors, and more.

***DHVI Flow Cytometry Shared Resource 2020 FlowJo Training Workshop, Day 2, Advanced: Recording posted here*** (Passcode is 1#AHfDKF)  Topics covered include advanced applications in FlowJo, including plugins and how to install them, data pre-processing features (such as DownSample, StainIndex, and concatenation), dimensionality reduction techniques (such as tSNE and UMAP), and clustering tools.

 

 

 

 

 

 

 

 

Facility Leadership

  • Director: Derek Cain, PhD
  • Flow Engineers: Steven Slater ASCP(SCYM) and Evan Trudeau ASCP(SCYM)
  • Research Technicians: Patti McDermott ASCP(SCYM) and Aria Arus-Altuz
  • Scientific Advisors: Gregory Sempowski, PhD and M. Anthony Moody, MD