The Flow Cytometry Facility instruments are capable of performing BSL-1 through BSL-3 live cell sorting, phenotypic acquisition, DNA cell cycle analysis, and intracellular marker analysis. Analytical and sorting capabilities up to 18 simultaneous parameters enable researchers to define subpopulations based on cell surface morphology as well as size and complexity. We have two cell sorters (BD FACS Arias) that are operated by facility staff. We have four cell analyzers (1 BD LSRII, 1 BD Fortessa,1 Luminex Muse and 1 iQue Screener PLUS) available 24/7 for trained users. Instruments are located in the RP105, MSRBII, and GHRB (RBL at Duke).
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DHVI Flow Cytometry Shared Resource 2021 FlowJo Virtual Training Workshop, Session 1 on Sept 29 at 10:00am, Intro to FlowJo v10: Registration link https://info.flowjo.com/duke-sept29 Learn how to use the FlowJo™ workspace, including how to load samples (experimental data), statistics, and gates, create groups and analyses, and generate tabular and graphical layouts. Designed for those new to FlowJo or who would like a refresher overview of the software
DHVI Flow Cytometry Shared Resource 2021 FlowJo Virtual Training Workshop, Session 2 on Sept 29 at 2:00pm, High Dimensional Data Analysis in FlowJo: Registration link https://info.flowjo.com/duke-sept29 As the number of dimensions being interrogated rises, thorough identification and characterization of populations using traditional manual gating analysis becomes more challenging. Join us to learn about the suite of tools in FlowJo™ that enable computational discovery in high parameter data sets.
Upcoming and Recorded Webinars
Intro to Cell Sorting Part I: https://www.youtube.com/watch?v=P6e9EbNdS64
Intro to Cell Sorting Part II: https://youtu.be/Y39MXA0Bmz4
Intro to Cell Sorting Part III: https://youtu.be/_Iy27WXf5Zk
Open Flow: Flourescence Compensation in Diva, reviews the principles behind compensation and how it is carried out on a BD Fortessa analyzer, using a live-run with a four color experiment. A recording of this session can be found on their YouTube.)
Open Flow: Multicolor Troubleshooting in Diva, focuses on rules of compensation and how our data can suffer when we do not follow them. A recording of this fourth session can be found on their YouTube.
- Director: Derek Cain, PhD
- Flow Engineers: Steven Slater, ASCP(SCYM) and Evan Trudeau, ASCP(SCYM)
- RTP Flow Facility Manager: Amanda Foreman, MS
- Research Technicians: Patti McDermott, ASCP(SCYM) and Aria Arus-Altuz
- Scientific Advisors: Gregory Sempowski, PhD and M. Anthony Moody, MD