Overview

The DHVI Viral Genetic Analysis Facility offers large-scale flourescent DNA sequencing, sequence analysis, and PCR purification to serve the viral genetic analysis needs of researchers at the Center for AIDS Research (CFAR), the Duke Human Vaccine Institute (DHVI), and the Duke community. The facility is equipped with two ABI 3730XL DNA analyzers, a 3100 Genetic Analyzer, a Beckman Coulter Biomek FXp Laboratory Automation workstation, an Eppendorf epMotion automated pipetting system, a PerkinElmer ScanArray Express, and a Genepix 4000 microarray scanner.  Our state of the art fluorescent DNA sequencing yields high-quality sequences of 800 or more bases per read.

In addition to basic sequencing, extensive analysis of sequence data is also offered.  These services include primer design, multiple sequence alignments, construction of phylogenetic trees, determination of genetic distances and mutation rates, recombinant viral genome analysis, and detection of drug resistance mutations.