Overview

The RBL: Immunology Unit is a service component of the Sempowski laboratory and the Regional Biocontainment Laboratory at Duke (housed in GHRB).  In addition to providing access to specialized instrumentation for Real-Time PCR, and Luminex-based multiplex assays, this unit assists investigators with the design and implementation of molecular, protein, and cellular assays to quantify immune reconstitution or immune responses in vitro and in vivo.  A major accomplishment of this facility has been the development of a patented Real-Time PCR assay system to quantify mouse T-cell receptor excision circles.  This assay has proven invaluable for monitoring thymopoiesis in mice.