Overview

The Immune Reconstitution and Biomarker Analysis Shared Resource is a service component of the Sempowski laboratory and the Regional Biocontainment Laboratory at Duke (GHRB).  In addition to providing access to specialized instrumentation for Real-Time PCR, and Luminex-based multiplex assays, this Shared Resource assists investigators with the design and implementation of molecular, protein, and cellular assays to quantify immune reconstitution or immune responses in vitro and in vivo.  A major accomplishment of this facility has been the development of a patented Real-Time PCR assay system to quantify mouse T-cell receptor excision circles.  This assay has proven invaluable for monitoring thymopoiesis in mice.